DNA

Part:BBa_K2486025

Designed by: Cau� Westmann, Gabriel Lencioni Lovate   Group: iGEM17_USP-Brazil   (2017-10-28)


16 base pair spacer

Usage and Biology

We create this spacer in order to optimize transcription terminator activity, the part was positioned between our coding squences and terminators. This part was based on an effort of [http://pubs.rsc.org/-/content/articlelanding/2017/mb/c6mb00789a/unauth#!divAbstract Rangel-Chavez et al (2016)] which proposes a consensus architecture for genetic circuits in synthetic biology and proposes that the distance from a stop codon and a terminator should be between 10 and 30 nucleotides. Further information about the uses of this part in our project can be seen in the following composite parts:

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None